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Adulterants interference on Fourier Transform Infrared analysis of raw milk Arq. Bras. Med. Vet. Zootec.
Ribeiro,D.C.S.Z.; Tavares,W.L.F.; Leite,M.O.; Cerqueira,M.O.P.; Lima,J.S.; Ferreira,L.F.; Feijó,F.A.C.; Haddad,J.P.; Fonseca,L.M..
RESUMO O objetivo deste trabalho foi analisar as leituras de composição do leite cru por meio de espectrofotometria FTIR, utilizando-se curva de regressão PLS, bem como as contagens de células somáticas e bacteriana total por citometria de fluxo, após adição de amido e sacarose. O leite cru foi adulterado com três concentrações de amido e sacarose (0,1%, 0,5% e 1%), colocado em frascos contendo bronopol ou azidiol, os quais foram armazenados em duas temperaturas (7±2°C e 25±2°C ). As análises foram realizadas após zero, três, 24, 48, 72 e 168 horas de armazenamento. O modelo de regressão linear múltipla foi utilizado para análise estatística. A adição de amido e sacarose resultou em mudança significativa (P<0,05) para todas as variáveis dependentes. O...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Raw milk; FTIR; Startch; Sucrose; Composition analysis.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000501649
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Bacillus cereus in Brazilian Ultra High Temperature milk Scientia Agricola
Pacheco-Sanchez,Cristiana de Paula; Massaguer,Pilar Rodriguez de.
Brazilian Ultra High Temperature (UHT) milk consumption has increased during the last decade from 187 to 4,200 million liters. In the continuous UHT process, milk is submitted for 2-4 s to 130-150ºC, in a continuous flow system with immediate refrigeration and aseptical packing in hermetic packages. This research had the purpose to verify the incidence of B. cereus species from the B. cereus group, in UHT milk. In 1998 high indexes of these organisms were reported, reaching 34.14% of the analyzed samples. Beyond this fact, there was the need to establish methods and processes adjusted for correct identification of B. cereus. Thus, commercial sterility tests of 6,500 UHT milk packages were investigated in two assays, after ten days incubation at 37ºC and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: FTIR; PCR; Commercial sterility; Statistical analyses.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162007000200008
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Biodegradation of erythrosin B dye by paramorphic Neurospora crassa 74A BABT
Jesus,Gisele Jane de; Corso,Carlos Renato; Campos,Adriana de; Franchetti,Sandra Mara Martins.
The present work used paramorphic forms of Neurospora crassa 74A to remove erythrosine. The fungus culture was grown in medium containing the dye, as only carbon source for 2 and 90 h of interaction. A washing process using distilled water isolated the cellular mass mycelia was dried for 12 h at 105ºC and transformed in fine powder and analyzed in FTIR. The supernatant was analyzed through spectrophotometer UV-Vis and FTIR. Significant differences in the spectrum of UV-VIS and FTIR were observed between the control and the supernatant and between wall control and the walls colored by red, in FTIR for 2 and 90 h. Some significant bands were modified, suggesting the possibility of enzymatic biodegradation in proportion to the time of contact between the dye...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biodegradation; FTIR; Neurospora crassa; Paramorphic; Erythrosin.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200028
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Bioflocculant production from Streptomyces platensis and its potential for river and waste water treatment BJM
Agunbiade,Mayowa; Pohl,Carolina; Ashafa,Omotayo.
ABSTRACT A bacterium isolated from Sterkfontein dam was confirmed to produce bioflocculant with excellent flocculation activity. The 16S rDNA nucleotide sequence analyses revealed the bacteria to have 99% similarity to Streptomyces platensis strain HBUM174787 and the sequence was deposited in the Genbank as Streptomyces platensis with accession number FJ 486385.1. Culture conditions for optimal production of the bioflocculant included glucose as a sole carbon source, resulting in flocculating activity of 90%. Other optimal conditions included: peptone as nitrogen source; presence of Mg2+ as cations and inoculum size of 1.0% (v/v) at neutral pH of 7. Optimum dose of the purified bioflocculant for the clarification of 4 g/L kaolin clay suspension at neutral...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Culture conditions Polysaccharide Flocculating activity Kaolin suspension EDX &amp; FTIR.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400731
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Caracterização estrutural e avaliação da eficiência de filtragem de velas cerâmicas porosas modificadas. Infoteca-e
ALES, C. R.; ASSIS, O. B. G..
Neste trabalho são apresentados os resultados parciais da alteração de velas comerciais pelo revestimento de SiO2 seguindo de tratamentos de hidrofilização e do depósito de enzimas. Caracteriza-se as superfícies filtrantes por técnicas microscópica de varredura eletrônica (MEV), e espectroscópicas de energia dispersiva de raios-X (EDX) e infravermelho com transformada de Fourier (FTIR) bem como, testes para avaliação da eficiência do sistema de filtragem em fluxo contínuo.
Tipo: Comunicado Técnico (INFOTECA-E) Palavras-chave: Microscopia de Varredura Eletrônica; MEV; Raio-X; EDX; FTIR.
Ano: 1999 URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/29481
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Caracterización óptica y estructural de nanopartículas de Allium sativum L. impregnadas en lomo de bovino Acta Agron. (Palmira)
Figueroa-López,Kelly Johana; Torres Vargas,Olga Lucía; Prías-Barragán,John Jairo; Ariza-Calderón,Hernando.
En el trabajo se determinaron las características ópticas y estructurales de nanopartículas de polvo de ajo (Allium sativum L.), obtenidas por reducción y selección de tamaño, para comparación con muestras de polvo de ajo comercial. El tamaño de partícula se determinó empleando microscopía electrónica de transmisión (TEM) y las caracterizaciones óptica y estructural se realizaron, respectivamente, por espectroscopia infrarroja transformada de Fourier (FTIR) y difracción de rayos X (DRX). Para determinar el efecto de las nanopartículas de polvo de ajo cuando fueron aplicadas sobre trozos de lomo (Longissimus dorsi) de bovino se utilizó la técnica de fluorescencia. Los tamaños de las partículas del polvo sometido a reducción y selección de tamaño presentaron...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ajo; Especia; Nanopartículas; TEM; DRX; FTIR; Fluorescencia; Lomo bovino.
Ano: 2015 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122015000100007
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Characterization of tropical starches modified with potassium permanganate and lactic acid BABT
Takizawa,Fabiano Franco; Silva,Graziela de Oliveira da; Konkel,Francisco Eneias; Demiate,Ivo Mottin.
In the present work some tropical starches were modified by an oxidative chemical treatment with potassium permanganate and lactic acid. The native and modified samples were evaluated by mid-infrared spectroscopy, differential dyeing, pH, expansion power, solubility and swelling power, clarity of the pastes, susceptibility to syneresis, carboxyl content and reducing power. All modified samples presented dark blue color, higher expansion power (except corn starch), carboxyl content and reducing power. The solubility of the modified starch granules was very high at 90ºC. At this temperature, it was not possible to measure their swelling power. The viscographic analysis showed decrease in peak viscosity and higher degree of cooking instability. The principal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Tropical starches; Oxidized starches; FTIR.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000600012
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Degradation of rubber to metals bonds during its cathodic delamination, validation of an artificial ageing test ArchiMer
Nakache, Maryline; Aragon, Emmanuel; Belec, Lenaik; Perrin, Francois-xavier; Roux, Gerard; Le Gac, Pierre-yves.
Rubber-metal assemblies used for submarine applications are often associated to cathodic protection which can lead to cathodic disboncling of the polymer/metal interface. The degradation mechanisms of polychloroprene/epoxy adhesive/copper-aluminium substrate systems associated to an accelerated ageing test are compared to the degradations resulting from natural ageing conditions in the Mediterranean sea. From destructives tests, a kinetic in four steps is identified for both ageing conditions. This kinetic is confirmed by Electrochemical Impedance Spectroscopy data during ageing. In order to further identify the failure mode, the disbondecl surfaces on polymer and steel sides are analyzed using SEM and FTIR. Both ageing conditions present the same type of...
Tipo: Text Palavras-chave: Ageing; Rubber to metal systems; Cathodic delamination; FTIR.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00056/16697/14370.pdf
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Exploring the Anticancer Activity of Grape Seed Extract on Skin Cancer Cell Lines A431 BABT
Mohansrinivasan,V.; Devi C.,Subathra; Deori,Meenakshi; Biswas,Ananya; Naine S,Jemimah.
In this study, grape seeds were extracted using ethyl acetate and petroleum ether by solvent-solvent extraction method. The phytochemical tests were performed to identify different phytochemical compounds present in the grape seed extract (GSE). Antibacterial activity of the GSE was determined using agar diffusion method against Gram- positive and Gram-negative bacteria. Gas chromatography-mass spectrometry (GC-MS) and Fourier transform infrared spectroscopy (FTIR) analysis was done to identify the presence of bioactive compounds and their functional groups. The GC-MS results revealed a total of four compounds, known to have potent activity against cancer cells, viz, squalene, the most potent compound found in ethyl acetate extract and diethyl phthalate,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: GSE (Grape seed extract); Phytochemical; FTIR; GCMS; Skin cancer cell line; Anticancer agents.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000400540
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Fourier Transform Infrared Spectroscopy (FTIR) for MUN analysis in normal and adulterated Milk Arq. Bras. Med. Vet. Zootec.
Oliveira,M.C.P.P.; Silva,N.M.A.; Bastos,L.P.F.; Fonseca,L.M.; Cerqueira,M.M.O.P.; Leite,M.O.; Conrrado,R.S..
The objective of this study was to evaluate the CombiScope FTIR equipment based on Fourier Transform Infrared methodology (FTIR), to assess the content of milk urea nitrogen (MUN) in Brazil. Repeatability and reproducibility of CombiScopeTM FTIR (Delta Instruments), and comparison with an enzymatic automated method (Chemspec® 150; Bentley Instruments) were tested to measure raw milk urea nitrogen (MUN). Additionally, MUN levels stability after storage of raw milk samples at 4ºC, and 20ºC for up to 15 days, and capability and precision to detect extraneous urea added as an adulterant to the milk were evaluated by FTIR equipment. There was a high correlation coefficient for the analysis of MUN by FTIR equipment, when compared with the automated enzymatic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Milk; Validation; Milk urea nitrogen; Infrared spectroscopy; FTIR.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000500037
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Impacto da queima nos atributos químicos e na composição química da matéria orgânica do solo e na vegetação PAB
Dick,Deborah Pinheiro; Martinazzo,Rosane; Dalmolin,Ricardo Simão Diniz; Jacques,Aino Victor Ávila; Mielniczuk,João; Rosa,Alessandro Samuel.
O objetivo deste trabalho foi avaliar o efeito residual de queimadas periódicas nos atributos químicos, teor e composição da matéria orgânica de um Latossolo Vermelho e na composição química da vegetação predominante. Os ambientes estudados foram: campo nativo pastejado, sem queima e sem roçada (PN); campo nativo queimado e pastejado (PQ); e mata nativa adjacente à pastagem (MN). As amostras de solo foram coletadas nas camadas 0-5, 0-20, 20-40 e 40-60 cm, para determinação dos atributos de fertilidade, teores de carbono e nitrogênio, e realização das análises de espectroscopia de infravermelho com transformada de Fourier (FTIR). A parte aérea da vegetação desses ambientes foi analisada por análise elementar e FTIR. A queima da pastagem reduziu os teores de...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aromaticidade; Composição elementar; FTIR; Mata nativa; Pastagem.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000500011
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Multivariate analysis of the spectroscopic profile of the sugar fraction of apple pomace BABT
Gabriel,Luciana Sutil; Prestes,Rosilene Aparecida; Pinheiro,Luís Antonio; Barison,Andersson; Wosiacki,Gilvan.
Pomace, the main by-product of apple juice processing, contains all the fruit's compounds such as minerals, sugars, fibers, enzymes and secondary metabolites after bioprocessing. Dried pomace from four apple varieties (Gala, Fuji, Catarina and Joaquina) was used to extract fructose, glucose, and sucrose, the main soluble sugars contained in apple pomace, to verify the possible use of the sugar fractions. The concentrated sugars were characterized by NMR and FTIR. The partial least squares method (PLS) applied to the NMR and FTIR spectra revealed large amounts of fructose, glucose, and sucrose, without the presence of others compounds. Principal component analysis (PCA) discriminated the studied apple varieties, with a 99% level of significance, as function...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Apple pomace; NMR; FTIR; Multivariate analysis.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000300012
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Optimization of Parameters for Biosynthesis of Silver Nanoparticles Using Leaf Extract of Aegle marmelos BABT
Christopher,JohnSamuel Godwin; Saswati,Banerjee; Ezilrani,PanneerSelvam.
ABSTRACTThe aim of this study was to optimize the biosynthesis of silver nanoparticles using leaves ofAegle marmelos as the primary source. The optimal reaction medium comprised 2:1 concentration of leaf extract and 6mM concentration of silver nitrate solution (pH 7. The biosynthesized silver nanoparticles were confirmed by UV-Vis spectroscopy at 420 nm, XRD and FTIR analysis. The antimicrobial properties of silver nanoparticles were confirmed withBacillus subtilis andPseudomonas aeruginosa.
Tipo: Info:eu-repo/semantics/article Palavras-chave: AgNO3; Aegle marmelos leaf; UV-Vis spectroscopy; XRD; FTIR.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500702
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Organic matter quality in a soil cultivated with perennial herbaceous legumes Scientia Agricola
Canellas,Luciano Pasqualoto; Espindola,José Antonio Azevedo; Rezende,Carlos Eduardo; Camargo,Plínio Barbosa de; Zandonadi,Daniel Basílio; Rumjanek,Victor Marcos; Guerra,José Guilherme Marinho; Teixeira,Marcelo Grandi; Braz-Filho,Raimundo.
Using herbaceous legumes in agricultural systems yields great quantities of plant residues, allowing changes in soil organic matter quality and content over the years. This study was conducted on an Ultisol, at Seropédica, RJ, Brazil, to evaluate the effects of different perennial herbaceous legumes on soil organic matter quality. A factorial scheme with three replications was used to evaluate the species: forage groundnut cv. BR-14951 (Arachis pintoi), tropical kudzu (Pueraria phaseoloides), and siratro (Macroptilium atropurpureum). After the first cut, each plot was divided into two subplots; plants were cut and left on the soil surface or cut and removed. Soil samples of a closed area covered by spontaneous vegetation (mainly C3 plants) or by Panicum...
Tipo: Info:eu-repo/semantics/article Palavras-chave: FTIR; Fluorescence; Humic acids; Humic substances; 13C.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162004000100010
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Separation of polyhydroxyalkanoates-producing bacterial strains using PHA synthase gene and their evaluation for PHA deposition BABT
Khan,Abdul Basit; Khattak,Mehmood Iqbal; Tarar,Omer Mukhtar; Habib,Farzana; Jamil,Khalid; Yasmin,Arfa; Parvez,Shoukat.
In this study, a variety of samples were screened for the presence of PHA synthase gene. Results showed that 16 out of 102 isolated were positive for PHA respective genes. The highest prevalence was observed in Pseudomonas aeruginosa. The capability of PHA production was also shown by growing these strains on the defined medium and subsequent analysis using intracellular granules staining and Fourier transform infrared spectroscopy (FT-IR). The microscopic analysis showed that the positive strains accumulated PHA in the cell. The FT-IR analysis showed the presence of PHA peaks in the dried cells as well as in extraction product. P aeruginosa strain P7 showed higher concentration of PHA compared to the others as demonstrated by the highest respective peaks...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Polyhydroxyalkanoates; Biodegradable polymers; PHA synthase gene; FTIR.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000400015
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Taxonomic Re-Investigation and Geochemical Characterization of Reid’s (1974) Species of Spiniferites from Holotype and Topotype Material ArchiMer
Gurdebeke, Pieter R.; Mertens, Kenneth; Bogus, Kara; Marret, Fabienne; Chomerat, Nicolas; Vrielinck, Henk; Louwye, Stephen.
The genus Spiniferites currently encompasses 142 dinoflagellate cyst species. Some Spiniferites species are difficult to identify because of an incomplete or doubtful description, and/or substandard iconography. This study re-describes and re-illustrates the Spiniferites holotypes first described by Reid in 1974 Reid PC. 1974. Gonyaulacacean dinoflagellate cysts from the British Isles. Nova Hedwigia. 25:579–637. [Google Scholar]. It also discusses topotype material from surface sediments recovered from British estuaries, and attempts to provide further constraints on the classification of species in this genus using the geochemical characterization of their cyst walls. Reid described four new Spiniferites species: Spiniferites belerius, Spiniferites...
Tipo: Text Palavras-chave: Dinoflagellate cyst; Redescription; Taxonomy; Geochemistry; Cyst wall composition; FTIR; Dinosporin.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00471/58306/60847.pdf
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The affiliation of Hexasterias problematica and Halodinium verrucatum sp. nov. to ciliate cysts based on molecular phylogeny and cyst wall composition ArchiMer
Gurdebeke, Pieter R.; Mertens, Kenneth; Takano, Yoshihito; Yamaguchi, Aika; Bogus, Kara; Dunthorn, Micah; Matsuoka, Kazumi; Vrielinck, Henk; Louwye, Stephen.
Species in the genera Hexasterias and Halodinium have been recorded over the last decades as acritarchs in palynological and/or plankton studies. In paleoenvironmental studies, these resting stages are often interpreted as indicators of freshwater input. The biological affinity of these genera has never been definitely established. Here, a new species, Halodinium verrucatum sp. nov., is described and molecular evidence (single specimen SSU and LSU rDNA sequencing) reveals that both this new species and Hexasterias problematica, collected from sediment samples in the Skagerrak and Baltic Sea, are resting stages of prorodontid ciliates. Additionally, infrared spectroscopic analysis (micro-FTIR) of Hexasterias problematica and Halodinium spp. specimens...
Tipo: Text Palavras-chave: Acritarch; Ciliate cyst; FTIR; LSU-SSU rDNA; Prostomatida; Taxonomy.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00468/57970/64186.pdf
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The use of chitosan in protecting wooden artifacts from damage by mold fungi Electron. J. Biotechnol.
El-Gamal,Rehab; Nikolaivits,Efstratios; Zervakis,Georgios I; Abdel-Maksoud,Gomaa; Topakas,Evangelos; Christakopoulos,Paul.
Background: Many buildings in Egypt e.g. museums, mosques and churches, do not possess controlled environments for minimizing the risks of damage of wooden artifacts due to the growth of fungi. Fungal damage usually appears as change in wood color, appearance of stains, and sometimes deformation of wooden surfaces. In this study we focused on the effect that some fungi exert on the properties of wooden artifacts and evaluated the effectiveness of different concentrations of chitosan on their protection against damage by mold fungi. Results: Samples were collected from different monuments and environments, and fungi growing on them were isolated and identified. The isolated Penicillium chrysogenum, Aspergillus flavus and /Aspergillus niger strains were used...
Tipo: Journal article Palavras-chave: Archeological wood; Chitosan protection; Crystallinity index; Damage of wooden artifacts; Filamentous fungi; FTIR; Fungal damage; Prevention of fungal growth; UV spectrophotometry; Wood deterioration; XRD.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000600011
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Variation analysis of bacterial polyhydroxyalkanoates production using saturated and unsaturated hydrocarbons BJM
Tufail,Saiqa; Munir,Sajida; Jamil,Nazia.
ABSTRACT Polyhydroxyalkanoates (PHA) are efficient, renewable and environment friendly polymeric esters. These polymers are synthesized by a variety of microbes under stress conditions. This study was carried out to check the suitability of waste frying oil in comparison to other oils for economical bioplastic production. Six bacterial strains were isolated and identified as Bacillus cereus (KF270349), Klebsiella pneumoniae (KF270350), Bacillus subtilis (KF270351), Brevibacterium halotolerance (KF270352), Pseudomonas aeruginosa (KF270353), and Stenotrophomonas rhizoposid (KF270354) by ribotyping. All strains were PHA producers so were selected for PHA synthesis using four different carbon sources, i.e., waste frying oil, canola oil, diesel and glucose....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Waste frying oil; Polyhydroxyalkanoates; Fluorescence microscopy; FTIR.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400629
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Vibrational and thermal characterization of seeds, pulp, leaves and seed oil of Rosa rubiginosa Boletín de la Sociedad
Ramos,Pablo Martín; Gil,Jesús Martín; Ramos Sánchez,M. Carmen; Navas Gracia,Luis Manuel; Hernández Navarro,Salvador; Martín Gil,Francisco Javier.
Rosa rubiginosa L. seed oil has been studied for its application in skin care products, but the chemical nature of seeds, pulp and even leaves, apart from that of oil, is also relevant with a view to the application of this weed for biodiesel production. All these vegetal materials were studied by infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC) for characterisation purposes. FTIR bands at 3005, 2924, 1740, 1654 and 1456 cm-1 were used to estimate the iodine index, suitable for biofuels, and the oxidation stability degree. From the viewpoint of the thermal stability, both the seed oil (for which pyrolysis occurs at 462ºC), the raw seeds and the rosehip pulp (with decomposition temperatures of 373ºC and 333ºC, respectively) showed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: A-linolenic acid; DSC; FTIR; Rose hip seed oil; R. rubiginosa.
Ano: 2016 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-23722016000300003
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